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. Author manuscript; available in PMC: 2018 Sep 13.
Published in final edited form as: Neuron. 2017 Sep 13;95(6):1292–1305.e5. doi: 10.1016/j.neuron.2017.08.039

Figure 2.

Figure 2

LPAC and QAGR RAN proteins accumulate in human DM2 brain. DM2 pre-mRNA and predicted RAN protein with underlined peptide used for antibody generation for sense (A) and antisense (B) RAN proteins. Antibody validation by detection of recombinant FLAG-tagged LPAC (C) or QAGR protein (D) by α-Flag and the respective custom antibody (α-LPAC or α-QAGR) by protein blot (left) or IF (right). Negative pre-immune controls are shown for IF. IHC staining of human DM2 and control autopsy tissue using α-LPAC (E) or α-QAGR (F) antibodies. At least three independent experiments were performed for panels C-D and panels E-F show examples based on the analysis of 4 DM2 and 3 control cases. See also Figure S2.