Figure 2.

CHE induced apoptosis in prostate cancer PC-3 cells.

Notes: Cells were treated with CHE at the indicated concentrations for 24 h in PC-3 cells. (A) Cell apoptosis determined by flow cytometry using Annexin V-FITC+PI staining. (B) Quantification of data presented in (A). (C) Western blot analysis for apoptosis-related markers in the PC-3 cells. The statistic data for apoptosis cells were indicated and presented as mean ± SE from three independent experiments. **p<0.01; all versus DMSO group.

Abbreviations: CHE, chelerythrine; FITC, fluorescein isothiocyanate; PI, propidium iodide.