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Flow diagram of preparation of CIK cells following two protocols (hydroxyethyl starch sedimentation or Ficoll-Hypaque density gradient centrifugation). The proliferation, phenotypes, cytokines secretion, and cytotoxicity of CIK cells in the two groups were evaluated.
Abbreviations: HES, hydroxyethyl starch; PB, peripheral blood; PBMC, peripheral blood mononuclear cell; CIK cells, cytokine-induced killer cells.
