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. 2018 May 8;11:2657–2672. doi: 10.2147/OTT.S162281

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© 2018 Shi et al. This work is published and licensed by Dove Medical Press Limited

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Phenotypes of CIK cells before and after ex vivo cell culture and induction. No significant differences were observed before ex vivo induction between purified and unpurified groups (A, purified group: n=25; unpurified group: n=21, P>0.05). After ex vivo induction, the percentages of CD8⁺, CD3⁻CD56⁺, and CD3⁺CD56⁺ cells in the unpurified group were significantly higher than those in the purified group (B, P<0.05). CIK cell phenotypes before and after culture were also compared in the two groups (C and D). The percentages of CD3⁺, CD8⁺, and CD3⁺CD56⁺ subsets were greatly increased after induction, and the percentages of CD4⁺, CD3⁻CD56⁺, and CD4⁺CD25⁺ cells were significantly decreased in both purified group (C) and unpurified group (D) (purified group: n=25; unpurified group: n=21, P<0.05). Compared between the two groups, *P<0.05.

Abbreviations: CIK cells, cytokine-induced killer cells; Pre-CIK, CIK precursor.