Figure 3. Ablation of NFκB in LEC results in decreased numbers of IL-17A- and GM-CSF-producing innate lymphocytes (see also Fig. S3).

(A–B) IL-17^creRosa26R^eYFP mice were infected i.t. with yeast. Lung cell suspensions were analyzed by flow cytometry at 48 h.p.i. to identify IL-17A⁺ innate lymphocytes. Cells were gated as CD90.2⁺IL-17⁺ and the proportion (A) and number (B) of nTh17, TCRβ⁺ CD4⁻, TCRγδ, and ILCs quantified. Total lymphocytes (B) means IL-17⁺ cells within the total CD90.2⁺ population. A representative of 3 experiments depicted; n=5/group. Mann-Whitney test (B). (C–D) IKK2^fl/fl and IKK2^ΔLEC mice were infected i.t. with yeast and the proportion (C) and number (D) of indicated lymphocyte populations quantified by flow cytometry at 48 h.p.i. The TCRβ⁺CD4⁻ gate includes CD8⁺ T cells. Concatenated plots depicted in C (n=11 in IKK2^fl/fl, n=9 in IKK2^ΔLEC). Two pooled experiments depicted in D; each symbol is one mouse. Mann-Whitney test.