Table 1.

Validation parameters for determination of triterpenic acids (n = 5).

Parameters	Oleanolic Acid	Ursolic Acid	Betulinic Acid
Concentration range	0.05–1.00 mg/mL	0.005–1.00 mg/mL	0.002–0.10 mg/mL
Correlation coefficient (r)	0.9994	0.9998	0.9999
Linear regression equation	y = 80,370,931x − 55,822	y = 132,468,713x − 13,814	y =101,240,680x − 28,874
RSD values of peak area	0.64–1.32%	0.83–1.22%	0.41–0.78%
LOD (µg/mL)	0.13	0.14	0.12
LOQ (µg/mL)	0.43	0.46	0.40