Figure 5. Generation of the tag fragment with custom sticky ends.

A. The sense annealing oligo (annealing oligo-S) is composed of 5’-AGCT (letters in red) and tag-specific sequence (letters in blue); the anti-sense annealing oligo (annealing oligo-AS) is composed of 5’-ATTC (letters in green) and tag-specific sequence (letters in blue), as shown in the right table. B. Both amplifying oligos contain a BsaI recognition site (letters in black), THSD1-specific sticky end (letters in red or green), and tag-specific sequences (letters in blue), as detailed on the right table.