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. 2018 Feb 5;8(2):e2721. doi: 10.21769/BioProtoc.2721

Figure 6. Schematic of making a tag clone from a template clone of THSD1.

Figure 6.

A-B. The template clone contains two THSD1-specific sticky ends (letters in red or green). C. All above tags containing a THSD1-specific sticky end and 5’-phosphorylation are joined with the linearized template clone by T4 DNA ligase, thus generating a seamless tag clone.