Table 5. Example parameters for multi-dimensional image acquisition.
| Dimensions | T’(140) x XY(16) x Z(2) x λ(4) | ||||
| Camera Name | Andor Zyla VSC-01632 | ||||
| 1.2 | |||||
| Numerical Aperture | 1.333 | ||||
| Number of Picture Planes | 4 | ||||
| Plane #1 | Plane #2 | ||||
| Name | Brightfield | Name | GFP | ||
| Component Count | 1 | Component Count | 1 | ||
| Modality | Widefield Fluorescence | Modality | Widefield Fluorescence | ||
| Camera Type | Andor Zyla | Camera Type | Andor Zyla | ||
| Binning | 2 x 2 | Binning | 2 x 2 | ||
| Exposure | 100 msec | Exposure | 150 msec | ||
| Readout Mode | Global shutter at 16-bit | Readout Mode | Global shutter at 16-bit | ||
| Readout Rate | 200 MHz | Readout Rate | 200 MHz | ||
| Conversion Gain | Dual Gain 1/3 | Conversion Gain | Dual Gain 1/3 | ||
| Spurious Noise Filter | on | Spurious Noise Filter | on | ||
| Sensor Mode | Normal | Sensor Mode | Normal | ||
| Trigger Mode | Internal | Trigger Mode | Internal | ||
| Temperature | -0.4 °C | Temperature | -0.4 °C | ||
| Microscope | Ti Microscope | Microscope | Ti Microscope | ||
| 2 (49002 - ET-GFP (FITC/Cy2)) | 2 (49002 - ET-GFP (FITC/Cy2)) | ||||
| 1 | Shutter, Shutter(EPI-BF) | Closed | |||
| Active | Shutter1, Shutter(EPI-FL) | Active | |||
| PFS-S, state | Off | PFS-S, state | Off | ||
| PFS-S, offset | 6729 | PFS-S, offset | 6729 | ||
| PFS-S, mirror | Inserted | PFS-S, mirror | Inserted | ||
| Zoom | 1.00x | ||||
| Plane #3 | Plane #4 | ||||
| Name | mKO | Name | E2Crimson | ||
| Component Count | 1 | Component Count | 1 | ||
| Modality | Widefield Fluorescence | Modality | Widefield Fluorescence | ||
| Camera Type | Andor Zyla | Camera Type | Andor Zyla | ||
| Binning | 2 x 2 | Binning | 2 x 2 | ||
| Exposure | 150 msec | Exposure | 150 msec | ||
| Readout Mode | Global shutter at 16-bit | Readout Mode | Global shutter at 16-bit | ||
| Readout Rate | 200 MHz | Readout Rate | 200 MHz | ||
| Conversion Gain | Dual Gain 1/3 | Conversion Gain | Dual Gain 1/3 | ||
| Spurious Noise Filter | on | Spurious Noise Filter | on | ||
| Sensor Mode | Normal | Sensor Mode | Normal | ||
| Trigger Mode | Internal | Trigger Mode | Internal | ||
| Temperature | -0.4 °C | Temperature | -0.4 °C | ||
| Microscope | Ti Microscope | Microscope | Ti Microscope | ||
| 1 (49014 - ET-mKO/mOrange) | 4 (49015 - Alexa 633) | ||||
| PFS-S, state | Off | PFS-S, state | Off | ||
| PFS-S, offset | 6729 | PFS-S, offset | 6729 | ||
| PFS-S, mirror | Inserted | PFS-S, mirror | Inserted | ||
| Time Loop | 140-Nonequidistant | ||||
| Z Stack Loop | 2 - Step 4 µm | ||||