| Substrate | Solvent | Concentration (10×)/Media | Final concentration |
Comment |
|---|---|---|---|---|
| Norepinephrine | - | 10 µM/ SH assay media | 1 µM | |
| FFA: BSA | RPMI no glucose | 4 mM/ RPMI no glucose | 0.4 mM; 4:1 | |
| Oligomycin | DMSO | 50 µM/ SH assay media | 5 µM | |
| Antimycin A | DMSO | 80 µM/ SH assay media | 8 µM | |
| FCCP | DMSO | 50 µM/ SH+ Na Pyruvate | 5 µM | |
| Na pyruvate | - | 100 mM/ SH assay media | 10 mM | pH=7.4 and filter |
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