Reagent | MW | Concentration | Solvent | Comments |
---|---|---|---|---|
Malate | 134.09 | 1.0 M | ddH2O | pH to 7.2 with KOH. Solution is strongly acidic, calculate molarity according to final volume. |
Succinate | 270.1 | 1.0 M | ddH2O | pH to 7.2 with KOH |
Oligomycin | 791.06 | 20 mM | DMSO | Complex V inhibitor |
Antimycin A | 548.63 | 40 mM | DMSO | Complex III inhibitor |
FCCP | 254.17 | 40 mM | DMSO | Chemical uncoupler |
Rotenone | 394.4 | 40 mM | DMSO | Complex I inhibitor |
Etomoxir | 338.76 | 40 mM | DMSO | CPT-1 inhibitor, blocks conversion of palmitoyl-coA to palmitoyl-carnitine |
ADP, K+ salt | 501.32 | Use as powder | ||
L-(−)-Carnitine | 161.2 | Use as powder |