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. Author manuscript; available in PMC: 2018 May 14.
Published in final edited form as: Bio Protoc. 2015 Nov 5;5(21):e1641. doi: 10.21769/BioProtoc.1641
Reagent MW Concentration Solvent Comments
Malate 134.09 1.0 M ddH2O pH to 7.2 with KOH. Solution is strongly acidic, calculate molarity according to final volume.
Succinate 270.1 1.0 M ddH2O pH to 7.2 with KOH
Oligomycin 791.06 20 mM DMSO Complex V inhibitor
Antimycin A 548.63 40 mM DMSO Complex III inhibitor
FCCP 254.17 40 mM DMSO Chemical uncoupler
Rotenone 394.4 40 mM DMSO Complex I inhibitor
Etomoxir 338.76 40 mM DMSO CPT-1 inhibitor, blocks conversion of palmitoyl-coA to palmitoyl-carnitine
ADP, K+ salt 501.32 Use as powder
L-(−)-Carnitine 161.2 Use as powder