Substrate | MW | 10× Conc. for port injection |
Additives (10× conc. for port injection) |
---|---|---|---|
Succinate | 270.1 | 100 mM | 40 mM ADP, 50 µM Rotenone |
Malate | 134.09 | 30 mM | 40 mM ADP |
Pyruvate | 110.94 | 100 mM | 40 mM ADP, 30 mM malate |
Palmitoyl-carnitine | 436.07 | 1 mM | 40 mM ADP, 30 mM malate, 5 mM carnitine |
Palmitoyl-CoA | 1,004.94 | 1 mM | 40 mM ADP, 30 mM malate, 5 mM carnitine |