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. Author manuscript; available in PMC: 2018 May 14.
Published in final edited form as: Bio Protoc. 2015 Nov 5;5(21):e1641. doi: 10.21769/BioProtoc.1641
Substrate MW 10× Conc. for
port injection
Additives (10× conc. for port
injection)
Succinate 270.1 100 mM 40 mM ADP, 50 µM Rotenone
Malate 134.09 30 mM 40 mM ADP
Pyruvate 110.94 100 mM 40 mM ADP, 30 mM malate
Palmitoyl-carnitine 436.07 1 mM 40 mM ADP, 30 mM malate, 5 mM carnitine
Palmitoyl-CoA 1,004.94 1 mM 40 mM ADP, 30 mM malate, 5 mM carnitine