Component	Reaction with [35S]methionine	Reaction without [35S]methionine
TNT® Rabbit Reticulocyte Lysate	50 µl	50 µl
TNT® Reaction Buffer	4 µl	4 µl
TNT® RNA Polymerase (T7)	2 µl	2 µl
Amino Acid Mixture, Minus Leucine, 1 mM	-	1 µl
Amino Acid Mixture, Minus Methionine, 1 mM	2 µl	1 µl
[35S]methionine (> 1,000 Ci/mmol at 10 mCi/ml)	4 µl	-
RNasin® Ribonuclease Inhibitor (40 U/μl)	2 µl	2 µl
DNA template(s) (1 μg/μl)	2 µl	2 µl
Nuclease-free dH2O	34 µl	38 µl
Total reaction volume	100 µl	100 µl