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. 2018 Apr 20;8(8):e2813. doi: 10.21769/BioProtoc.2813
Component Volume
NEBuffer 3 (10x) 1 µl
25x RNase-free PI (25x) 0.4 µl
RNasin RNase Inhibitor (40 U/µl) 0.2 µl
RRL translation reactions (µl) 3 µl
Nuclease-free dH2O (µl) 4.4 µl
CIAP (10 U/µl) 1 µl
Total (µl) 10 µl