Component | Volume |
---|---|
NEBuffer 3 (10x) | 1 µl |
25x RNase-free PI (25x) | 0.4 µl |
RNasin RNase Inhibitor (40 U/µl) | 0.2 µl |
RRL translation reactions (µl) | 3 µl |
Nuclease-free dH2O (µl) | 4.4 µl |
CIAP (10 U/µl) | 1 µl |
Total (µl) | 10 µl |
Component | Volume |
---|---|
NEBuffer 3 (10x) | 1 µl |
25x RNase-free PI (25x) | 0.4 µl |
RNasin RNase Inhibitor (40 U/µl) | 0.2 µl |
RRL translation reactions (µl) | 3 µl |
Nuclease-free dH2O (µl) | 4.4 µl |
CIAP (10 U/µl) | 1 µl |
Total (µl) | 10 µl |