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. 2018 Apr 14;11(4):605. doi: 10.3390/ma11040605

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PC12 cell viability and differentiation on HTE and AR Ti surfaces. (A) Confocal laser scanning micrographs showing viable (green) and nonviable (red) bacterial cells on the HTE- Ti and AR-Ti surfaces. The majority of PC12 cells still survived on both surfaces after one day of incubation, and the mechanobactericidal surface of HTE-Ti did not have any killing effect towards PC12 cells (scale bar 50 µm). (B) Day 1, PC12 cell attachment and proliferation on HTE-Ti and AR surfaces. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) results suggest that the HTE-Ti facilitated cell attachment. No differences were detected in the total protein content (BCA, bicinchoninic acid protein assay) of the two samples tested. (C) PC12 cell differentiation on HTE-Ti. PC12 cells present on HTE-Ti surface exhibited enhanced neurite growth for 7 days. The cells grown on the AR surfaces were not able to undergo differentiation.