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. 2018 May 2;14(5):e1007048. doi: 10.1371/journal.ppat.1007048

Fig 2. Generation and characterization of phospholipid-bilayer nanodiscs containing receptor molecules.

Fig 2

A Size exclusion chromatography (SEC) elution profiles (absorption at 227 nm over elution volume Ve) for nanodiscs containing either no receptor molecules (empty), GT1b only, GST-Syt-II 1–90 only, or GT1b and GST-Syt-II 1–90 (dual-receptor nanodiscs; schematic inserts modified from [73]). B ELISA results for the detection of membrane scaffold protein (MSP; via His-tag), GT1b, and Syt-II in the SEC-fractions. Brackets embrace the fractions used for the SPR measurements or further purification using the GST-tag on Syt-II (marked by asterisk). C Electron microscopy of the assembled nanodiscs purified by SEC (empty and GT1b nanodiscs) or SEC and batch-purification via the GST-tag (Syt-II nanodiscs and dual-receptor nanodiscs). Arrows indicate assembled discoidal nanodiscs of the correct diameter (13 nm) from top view. Scale bar = 50 nm.