Fig 3. Kinetic analysis employing nanodisc-embedded receptor molecules reveals that interactions with gangliosides, proteins, and lipids are crucial for high-affinity binding.

SPR-sensorgrams for the determination of binding kinetics of recombinant H_C receptor-binding domains to isolated GST-Syt-II 1–61 (A), GT1b nanodiscs (B), GST-Syt-II 1–90 nanodiscs (C), or dual-receptor nanodiscs containing both GT1b and GST-Syt-II 1–90 (D). Shown is one representative of two measured binding responses (red) overlaid with fits (black lines) from 1:1 Langmuir interaction models or a heterogeneous binding model (marked by asterisk), see Table 1 for kinetic data.