Fig 4. Membrane integration of the H_C loop compensates for the entropic penalty inflicted by dual-receptor binding at physiological temperatures.

A Representative SPR-sensorgrams for determination of thermodynamic binding parameters for H_CB wild-type or the ΔH_C loop mutant to isolated Syt-II, Syt-II nanodiscs, or dual-receptor nanodiscs measured at the temperatures indicated in the bar above the graphs. B. Thermodynamic profile depicting binding enthalpy (ΔH°), entropy (-TΔS°), and the standard Gibbs free energy (ΔG°) for those interactions; see also Table 2 for thermodynamic data.