. 2018 May 2;14(5):e1007048. doi: 10.1371/journal.ppat.1007048

Table 2. Thermodynamic binding parameters of H_CB and H_CB ΔG1247-F1250 (mean ± SD of n = 4 (H_CB—Syt-II) or n = 2 (others) replicate measurements).

Interaction	ΔH°(kJ/mol)	–TΔS° (kJ/mol at 25°C)	ΔG° (kJ/mol at 25°C)	K_D (nM at 25°C)^b
H_CB—Syt-II^a	-30.9	-11.2	-42.2	34
H_CB—Syt-II	–30.3 ±0.7	–7.9 ±0.7	–38.27 ±0.02	190 ±15
H_CB—Syt-II nanodiscs	–58.4 ±0.2	18.99 ±0.03	–39.4 ±0.2	140 ±14
H_CB ΔG1247-F1250 –Dual-receptor nanodiscs	–61 ±10	22 ±10	–39.5 ±0.4	120 ±31
H_CB—Dual-receptor nanodiscs	–14 ±10	–32 ±9	–47.8 ±0.1	4.4 ±0.3

^a For comparison: values taken from [24].

^b For comparison: affinity data at 25°C. Full kinetic and affinity data shown in supporting Table 1.

Table 2. Thermodynamic binding parameters of HCB and HCB ΔG1247-F1250 (mean ± SD of n = 4 (HCB—Syt-II) or n = 2 (others) replicate measurements).