Fig 5. Infection with PruQ-Δap2IV-4 tachyzoites preferentially recruits inflammatory monocytes.

(A.) Peritoneal exudate cells were harvested at day six post-infection from mice inoculated with either PruQ-parent, PruQ-Δap2IV-4, or PruQ-Δap2IV-4:AP2IV-4 strains and subjected to immunostaining for flow cytometry. Cells were stained for extracellular antigens, and gated on CD45+ cells for analysis. Inflammatory monocytes (Ly6C^hi, Ly6G^neg) and neutrophils (Ly6C^int Ly6G^hi) were determined and averaged (B.) Representative cytospin images are provided immediately below the matching FACS plots. (C.) Total cell infiltration into the peritoneum of infected mice. Cell counts were quantified based on volume recovered from the peritoneal wash. (D.) Absolute cell quantification of inflammatory monocytes and neutrophils. Data are representative of a minimum of 4 individual experiments and 5 mice per group. Statistical significance (*, p<0.05; **, p<0.01) is indicated.