Skip to main content
. Author manuscript; available in PMC: 2019 Apr 19.
Published in final edited form as: Cell. 2018 Apr 19;173(3):762–775.e16. doi: 10.1016/j.cell.2018.03.076

Figure 3. GPR68 is necessary for shear stress-induced calcium transients in MDA-MB-231 cells.

Figure 3

(A)The response of MDA-MB-231 cells after treatment of siRNAs against the indicated candidates. ** p<0.01 (B) Quantification of the MDA-MB-231 cells’ response to shear stress after treatment with smartpool siRNA oligos and the individual siRNA oligos against GPR68. ** p<0.01. (C) Response of MDA-MB-231 cells to proton stimulation at various final pH. The cells were incubated with HBSS at pH7.4 before the addition of the HBSS with various acidity. n=3. (D) The response of MDA-MB-231 cells to proton stimulation (final pH 6.5) after knocking down GPR68 by siRNA smartpool. (E) The response of MDA-MB-231 cells to shear stress stimulation in buffers with various pH. The cells were incubated with assay buffer at pH7.4. The pH was then changed by adding buffer with various acidity 3 min prior to the onset of shear stress stimulation.