Figure 6.
Myeloid cells in the amniotic fluid during the second and third trimester. (A) Flow cytometry gating strategy for immunophenotyping of myeloid cells. Cells were initially gated within the viability gate and CD45+ gate followed by CD15 and CD14 gating. (B) The numbers of neutrophils in the amniotic fluid of women at 15-20, 20-30, 30-36, or 37-40 weeks of gestation. (C) The numbers of monocytes/macrophages in the amniotic fluid of women at 15-20, 20-30, 30-36, or 37-40 weeks of gestation. n=6-34 per group.