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. Author manuscript; available in PMC: 2018 Aug 28.
Published in final edited form as: Nature. 2018 Feb 28;556(7699):57–63. doi: 10.1038/nature26155

Extended Data Figure 3. Transcriptional activation of reporter site PAM libraries with xCas9.

Extended Data Figure 3

Transcriptional activation by dSpCas9–VPR and dxCas9–VPR variants, transfected as plasmids, on GFP reporter plasmids containing different PAM sites in HEK293T cells. a, Earlier generations of xCas9 variants were tested on the R1 site with the NGG, NNN, or NNNNN PAM libraries. Values and error bars reflect the mean and s.d. of n=3 biologically independent samples. b, c, Transcriptional activators dxCas9(3.6)–VPR and dxCas9(3.7)–VPR were tested on two different protospacer reporters, R1 in (a) and R2 in (b), containing adjacent NGG, NNN, or NNNNN PAM libraries. Values and error bars reflect the mean and s.d. of n=3 biologically independent samples.