Skip to main content
. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Brain Struct Funct. 2017 Aug 3;223(1):233–253. doi: 10.1007/s00429-017-1487-y

Figure 2. RGS14 antibody specifically recognizes RGS14, but not other RGS proteins.

Figure 2

Lysates from hippocampus or HEK 293 cells transfected with RGS2, 4, 10, 12, 14, or 16 were immunoblotted for recognition by the RGS14 antibody or their respective tag. Lane 1: The RGS14 antibody recognizes a band corresponding to full-length RGS14 in the mouse hippocampus. Lanes 2–7: FLAG-RGS14, FLAG-RGS10, GFP-RGS12 trans-spliced (TS), RGS2-HA, RGS4-HA, and RGS16-HA were immunoblotted for RGS14 (top), or their respective tags (bottom). The RGS14 antibody recognized full-length, recombinant FLAG-RGS14 (lane 2), but not the other RGS (lanes 3–7), including RGS10 and RGS12, which are in the same R12 family as RGS14 and thus have a closely related sequence. Immunoblotting for each respective tag confirmed expression of each construct.