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Schematic representation of the In-fusion cloning steps for the generation of (A) Simian Human Immunodeficiency Virus (SHIV) and (B) Simian tropic Human Immunodeficiency Virus (stHIV). Inverese PCR was used for the linearization of the vector backbone, pSHIVAD8-EO. HIV env gene specific primers were used for the amplification of the each env from specific HIV infectious molecular clones as described previously.
