Fig 4. Replication kinetics of newly generated SHIVs in rhesus macaque PBMCs.

Pooled PBMC from randomly selected naive RM donors were stimulated with concanavalin A (Con-A) for 48-72hrs washed and infected with 200TCID₅₀ of various SHIVs as described in the method. Culture supernatents were harvested every three days upto 20-25 days and SIV gag protein (P27) were measured using P27 ELISA kits. Left panel represents a replication kinetics of full-length SHIVs as well as S375H/Y mutation and right panel represents truncated SHIVs and S375H/Y mutation.