Fig 7. CLCA2 and TMEM16A do not directly interact.

(A) Immunoblots of cells transfected with CLCA2, TMEM16A, or both. Proteins were immunoprecipitated and detected with antibodies specific for either protein. (B) Cells stably expressing vector or CLCA2 were transiently transfected with TMEM16A (TMEM) and treated 48h later with the protein cross-linker DSS, followed by immunoprecipitation and immunoblot. TMEM16A was detected with anti-Flag tag antibody, and CLCA2 was detected using TVE20 antibody. In indicated experiments, cells were treated with 1 micromolar ionomycin 5min before adding cross-linker. 1, position of protein monomer. For CLCA2, this includes the 130 kDa precursor and the 100 kDa N-terminal product. 2, apparent multimers. Right, size marker positions are indicated.