Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Danio rerio) | TRPC4DR | N/A | NCBI Reference sequence: NM_001289881 | Genes ordered from GenScript |
| Cell line (HEK293 GnTI-) | HEK293 GnTI- | ATCC | CRL-3022 | |
| Cell line (HEK293) | HEK293 | ATCC | CRL-1573 | |
| Cell line (Sf9) | Sf9 | N/A | Cat.No.600100 | Purchased from Oxford Expression Technologies Ltd (UK) |
| Recombinant DNA reagent | pcDNA3.1+TRPC4DR(See Materials and methods section for details) | This paper | Cloned by GenScript in the customized pcDNA3.1 vector harbouring Nterm-HIS8tag-TEV- and Cterm-HRV3C-eGFP | |
| Recombinant DNA reagent | pEG BacMam | Eric Gouaux Lab PMID: 25299155 | ||
| Recombinant DNA reagent | pEG BacMam +TRPC4DR(See Materials and methods section for details) | This Paper | Subcloned the pcDNA construct harbouring His-TEV- TRPC4- HRV3C -eGFP into the pEG BacMam with the introduction of StrepII tag. | |
| Chemical compound, drug | (-)Englerin A | N/A | Obtained from Lead Discovery Center GmbH, Dortmund Germany | |
| Software, algorithm | SPHIRE software package | Moriya et al., 2017 PMID: 28570515 | ||
| Software, algorithm | CrYOLO | N/A | Wagner et al., unpublished. manuscript in preparation | |
| Software, algorithm | RosettaCM | Wang et al. (2015) PMID: 27572730 | ||
| Software, algorithm | Rosetta | Wang et al. (2015) PMID: 27572730 | ||
| Software, algorithm | rosettaES | Frenz et al. (2017) PMID: 28628127 | ||
| Software, algorithm | Chimera | Pettersen et al. (2004) PMID:15264254 |