Table 1. EM data collection and refinement statistics of TRPC4DR.
| Data collection | Data set 1 [Data set 2] |
|---|---|
| Microscope | Titan Krios (Cs corrected, XFEG) |
| Voltage (kV) | 300 |
| Camera | K2 summit (Gatan) |
| Pixel size (Å) | 1.09 [1.09] |
| Number of frames | 60 [40] |
| Total electron dose (e-/Å2) | 69 [74] |
| Number of particles | 132,622 |
| Estimated defocus range | 0.844–2.931 |
| Atomic model composition | |
| Non-hydrogen atoms | 21,412 |
| Protein atoms | 21,192 |
| Ligand atoms | 236 |
| Refinement (Phenix) | |
| RMSD bond | 0.01 |
| RMSD angle | 1.11 |
| Model to map fit, CC mask | 0.80 |
| Resolution (FSC@0.143, Å) | 3.6 |
| Map sharpening B-factor (Å2) | −130.0 |
| Validation | |
| Clashscore | 3.53 |
| Poor rotamers (%) | 0.17 |
| Favoured rotamers (%) | 94.48 |
| Ramachandran outliers (%) | 0.0 |
| Ramachandran favoured (%) | 92.38 |
| Molprobity score | 1.62 |
| EMRinger score | 2.42 |