Table 3.
Rotational diffusion parameters of villin at different concentrations
| [villin] (mM) |
Np |
τRf (ns) |
τRf−1 (ns−1) |
τRs (ns) |
τRs−1 (ns−1) |
SR2 |
τ (ns−1) |
τini (ns−1) |
Dr (ns−1) |
Dr,ini (ns−1) |
Dr slow-down | Dr, ini slow-down |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dilute | 1 | 0.59 (0.08) |
1.870 (0.321) |
1.48 (0.45) |
0.887 (0.181) |
0.49 (0.13) |
0.820 (0.026) |
0.722 (0.022) |
0.204 (0.006) |
0.232 (0.007) |
1 | 1 |
| 8 | 8 | 0.97 (0.13) |
1.142 (0.110) |
3.96 (0.93) |
0.342 (0.052) |
0.17 (0.03) |
1.388 (0.180) |
1.095 (0.128) |
0.134 (0.014) |
0.166 (0.015) |
0.65 | 0.72 |
| 16 | 8 | 1.00 (0.09) |
1.069 (0.099) |
3.80 (0.21) |
0.271 (0.016) |
0.42 (0.05) |
2.223 (0.207) |
1.489 (0.118) |
0.082 (0.009) |
0.119 (0.011) |
0.41 | 0.51 |
| 32 | 8 | 1.83 (0.20) |
0.601 (0.065) |
13.81 (0.81) |
0.075 (0.005) |
0.52 (0.06) |
8.151 (0.910) |
3.574 (0.487) |
0.024 (0.004) |
0.057 (0.010) |
0.12 | 0.25 |
| 32 | 64 | 2.70 (0.15) |
0.445 (0.023) |
24.50 (1.07) |
0.047 (0.003) |
0.54 (0.02) |
14.445 (0.721) |
5.550 (0.369) |
0.014 (0.001) |
0.038 (0.002) |
0.07 | 0.16 |
Fast (τRf) and slow (τRs) correlation times and SR2 weight as in Eq. 8. Averaged inverse tumbling times are reported as well. Total tumbling times τ and τini were obtained according to Eqs. 10 and 11. Rotational diffusion coefficients Dr and Dr,ini were obtained according to Eq. 9. Error estimates are given in parentheses and the slow-down is relative to rotation in dilute solvent.