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. 2018 May 14;9:1892. doi: 10.1038/s41467-018-04231-7

Fig. 5.

Fig. 5

Truncation of Srv2/CAP’s C-terminal tail severely compromises actin cytoskeleton. a Western blots showing Srv2 expression levels in yeast strains with tubulin immunoblotting and Ponceau staining as loading controls. See Supplementary Fig. 7 for uncropped images. b Five-fold serial dilutions of yeast strains grown on YEPD plates at the indicated temperatures. c Structured illumination microscopy (SIM) of filamentous actin networks in yeast cells. Cells were grown in YEPD at 25 °C, fixed, and stained with Alexa488-phalloidin; scale bar, 5 µm. d Quantified differences in actin patch number and cell size across indicated yeast strains; n = 20 cells per strain; One-way, non-parametric ANOVA, p < 0.01 (**) and p < 0.0001 (****)