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. 2018 May 14;9:1879. doi: 10.1038/s41467-018-04286-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Two-photon imaging of subcellular activity in layer 1 of the M1 during the task. a Representative time-averaged two-photon image of dendritic compartments at a depth of 51 μm from the cortical surface in the M1 of marmoset D. Scale bar, 30 μm. b Motion-corrected ΔF/F traces of three representative dendritic compartments indicated by the numbers in a. The Y-axis trajectory of the pole is shown at the bottom. c Map of correlation coefficients between the Y-axis cursor trajectory and the ΔF/F trace in each pixel. d Map of correlation coefficients in the ΔF/F traces with ROI 1 (left), ROI 2 (middle), and ROI 3 (right). e Representative time-averaged two-photon image of axonal buttons at a depth of 46 μm in the M1 of marmoset D. Scale bar, 20 μm. Other conventions are the same as in a. f Motion-corrected ΔF/F traces of two representative axonal buttons indicated by the numbers in e. g Map of correlation coefficients between the Y-axis cursor trajectory and the ΔF/F trace in each pixel. h Maps of correlation coefficients for the fluorescence changes for ROI 1 (left), and ROI 2 (right)