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. 2018 May 14;8:7503. doi: 10.1038/s41598-018-25918-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microarray displaying the whole-proteome of Ct. (a) Results of the determination of on-chip protein expression using antibody staining against the terminal tags (green signal: anti-V5 antibody; red signal: anti-His antibody; yellow signal: overlay of both signals). All 898 proteins were spotted onto one array. The last 22 spots indicated by red and white frames represent the negative controls NC1 and NC2, respectively. The upper part of the array comprised genes of interest (GOI) with a DNA template length of up to 900 bp. (b) Final MFI values obtained by the anti-V5 antibody were sorted in descending order and plotted against the overall number of Ct proteins. The calculated cut-off (100 MFI) is represented by a red line, indicating that less than 10% of all proteins are below the cut-off and are therefore considered not to be expressed in full length.