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. 2018 May 14;8:7503. doi: 10.1038/s41598-018-25918-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proteome Immunoassays (PIA) using pools of five sera. In total 897 Ct proteins and one Cp protein were spotted on one array. (a) No signal was obtained with a pool of five sera from Ct-uninfected women. (b) Result of PIA with a pool of 5 sera from cancer-free women infected with Ct and <25 years of age. (c) Same as b, but women >40 years of age. (d,e) Results of PIAs with two different pools of samples from Ct-infected cervical cancer patients. Examples for antigens associated with general Ct infections (b–e), potentially persistent infections (c–e) and cervical cancer (d,e) are highlighted with green, yellow, and blue boxes, respectively.