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. 2018 May 14;8:7503. doi: 10.1038/s41598-018-25918-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Performance of de novo identified antigens in multiplex serology. Two Ct infection reference groups (grey, positive, and white, negative) were analyzed with four antigens representing possible new serological Ct infection markers. On the y-axis, MFI are plotted on a logarithmic scale, and distribution of reactivities for each group and antigen is displayed as box plot. Cut-off values were determined by Receiver Operating Characteristic (ROC) analysis and are shown as red lines resulting in specificity and sensitivity estimates shown in the inserted table.