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. 2018 May 14;9(5):577. doi: 10.1038/s41419-018-0529-6

Fig. 5. E2F7 effects on protein synthesis can be dissociated from cell cycle.

Fig. 5

a U2OS cells treated with either control (GFP) or E2F7 siRNA for 72 h. Cells were grown under starvation conditions for 4 h before being re-fed with full growth medium, DMEM (re-feed) for 1 h before being harvested for FACS analysis. Cells were set up in triplicate. b Puromycin incorporation assays were carried out on U2OS cells grown under starvation conditions for 4 h before being re-fed with full growth medium, DMEM (re-feed) for 1 h. Graph represents fold puromycin incorporation after normalising to actin levels (GFP EBSS control set as 1). n = 2 independent experiments