Fig. 5.

Linking vesicles over long distances through tethers. a Tethers between two vesicles were formed when one vesicle was pulled relative to another stationary surface-adhered vesicle. White arrow indicates direction of pulling. Labelling each vesicle with a different fluorophore (Rh-PE = purple; NBD-PE = blue) demonstrates the existence of two sub-tethers, one from each vesicle, which met at the middle and anchored on a sub-micron adhesion patch. b The precise location of the anchor point (yellow arrow) could be varied by extending the tether by pulling the vesicle. Dotted white circles represent location of a non-fluorescence vesicle. Scale bar = 10 µm for all images