Fig. 3.

Tension in F-actin rings around the microgel is responsible for compression. a Representative fluorescent images show that treatment with Latrunculin A (1 μM final concentration) gradually dissipated F-actins (red) around the microgel, followed by the expansion of the microgel (green). b The corresponding bright field images of the embedded microgel (Young’s modulus of 1440 Pa) in multilayered cells. c 3D total traction maps and its mean value calculated with respect to its reference time at 90 min. The 3D traction maps show that the microgel was gradually decompressed as the filamentous actins disappeared. All scale bars, 10 µm