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. 2018 May 15;4:23. doi: 10.1038/s41421-018-0020-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a RT-PCR analysis of hepatocytic specific markers for differentiated MHPCs. MHPC-Hep refers to MHPC-derived hepatocytes. β-actin was used as a loading control. b Ultrastructure of MHPC-derived hepatocytes. Arrowheads indicate bile canaliculi (Bc) and endoplasmic reticulum (ER); arrows indicate tight junction (Tj), glycogen granules (Gly), and mitochondria (M) (scale bar = 1 µm). c In vitro functional evaluation of MHPC-derived hepatocytes, including (i) indocyanine green (ICG) uptake, (ii) PAS staining for glycogen storage, and (iii) Oil Red O staining for lipid accumulation. d In vitro bipotency of MHPCs, including (i) hepatic differentiation with 20 ng/ml OSM induction on 2D matrigel showed doughnut-like hepatocyte cluster morphology, and (ii) PAS staining for glycogen storage in MHPC-derived hepatocytes, (iii) branching structure of cholangiocytes formed by culturing in 3D type 1 collagen gel culture system, and (iv) CK19 staining (scale bar = 100 µm)