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. 2018 May 15;4:23. doi: 10.1038/s41421-018-0020-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a H&E and Sirius Red staining of liver tissues from normal and 0.1% DDC-treated nude mice. Numerous small bile ducts (BD) appeared around the portal veins (PV) in the DDC liver (arrows). CV refers to central vein. b Serial immunostaining of GFP and CK19 in liver tissues from transplanted DDC-injured nude mice. The results showed the EGFP-positive MHPCs integrated in the large and small BD and expressed cholangiocyte marker CK19 (scale bar = 100 µm). c Sequence analysis of genetically modified MHPCs with CRISPR/Cas9 system for clone 1. Clones 1 and 2 showed identical deletions. WT refers to wild-type MHPCs. Two nonsense point mutations (A-C) and (C-G) were generated in the marmoset p53 gene. d Western blot analysis for the expression of p53 in transfected MHPCs. GAPDH was used as a loading control. A unit of 130 µg total protein was loaded. e Wound-healing assay for examining the migration rate of p53^−/− MHPCs. A significantly faster migration rate was detected in p53^−/− MHPCs than WT MHPCs (n = 5, two-tailed t-test, **P < 0.01)