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. 2018 May 14;9:1884. doi: 10.1038/s41467-018-04271-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Vaccine-elicited antibodies cross-neutralize Machupo virus. a Pseudotypes for the indicated arenaviruses were pre-incubated with monoclonal antibodies CR1-28 (left panel) or CR1-07 (right panel) at 100 µg ml⁻¹. Entry levels measured as in Fig. 1f. LASV pseudotype is included as a control. b Left: ELISA of CR1-28 binding to immobilized JUNV GP1 or MACV GP1. The calculated EC₅₀ value is shown in parentheses. LUJV GP1 is a control. Right: HEK293T cells were challenged with JUNV or MACV pseudotypes after pre-incubation with CR1-28 at the indicated concentrations. The calculated IC₅₀ value is shown in parentheses. VSIV pseudotype is included as a control. c Left: ELISA of CR1-07 binding to immobilized JUNV GP1 or MACV GP1. The calculated EC₅₀ value is shown in parentheses. LUJV GP1 is a control. Right: HEK293T cells were challenged with JUNV or MACV pseudotypes after pre-incubation with CR1-07 at the indicated concentrations. The calculated IC₅₀ value is shown in parentheses. VSIV pseudotype is included as a control. d Sensorgrams for binding of CR1-07 Fab binding to immobilized MACV GP1 as measured by surface plasmon resonance. The recorded sensorgrams (one of duplicates) are shown. The calculated K_D value is shown in parentheses. e Left: Percent plaque number (relative to a no antibody control) for infectious JUNV (Romero strain) with CR1-28 or CR1-07 at the indicated concentrations. Right: Percent plaque number (relative to a no antibody control) for infectious MACV (Carvallo strain) with CR1-28 or CR1-07 at the indicated concentrations. 17b is an HIV-1 neutralizing monoclonal antibody used as control. Error bars indicate S.D. (for some data point, error bars are smaller than symbols). For ELISAs, the experiment was performed twice in duplicate, and representative data are shown. For pseudotype neutralization studies, data are averaged from two independent experiments performed in duplicate. For studies with authentic viruses, the experiment was performed once in duplicate.