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. 2018 May 14;9:1884. doi: 10.1038/s41467-018-04271-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Sequence diversity in New World arenavirus GP1s. a Overlay of ribbon diagrams of JUNV GP1 (red, PDB: 5EN2¹⁴) and MACV GP1 (gray, PDB: 3KAS¹³). N-linked glycans are shown as sticks. Asterisks indicate N-linked glycans that differ in position between JUNV and MACV. Green spheres are sites of substitutions between the JUNV MC2 strain and Candid #1, the live attenuated vaccine strain. Loop 10 is a disulfide-linked insert that is unique to MACV. Disulfide bridges are shown as yellow sticks. b Sequence alignment of New World arenavirus GP1s. Antibody and TfR1 contacts are indicated. Substitutions in Candid #1 (with respect to JUNV MC2) are highlighted in green. Tree diagrams indicate predicted sites of N-linked glycosylation for JUNV GP1. The figure was generated using ESPrit3⁶⁴ and modified. GenBank accession numbers used for sequences used for the alignment are JUNV (MC2, D10072), JUNV (Candid #1, ACY70854.1), MACV (Carvallo, NC_005078), GTOV (INH-95551, NC_005077), CHPV (810419, YP_001816782.1), and SBAV (SPH114202, NC_006317). The sequence of TCRV shown in the alignment is that of the pseudotype used in this study and other studies,^{14, 30} which has a deletion spanning amino acid residues 121–132 and three substitutions (I134A, G418S and E458R) compared to GenBank NC_004293. c Surface representation of JUNV GP1 (PDB: 5EN2)¹⁴ showing conservation based on the alignment with MACV GP1 shown in b generated with ESPrit3.⁶⁴ Dashed circle indicates the location of the Tyr211_TfR1 pocket. d Surface representation of JUNV GP1 (PDB: 5EN2)¹⁴ showing sequence conservation with MACV, GTOV, CHPV, SBAV as shown in b. Using the algorithm available in ESPrit3,⁶⁴ residues with a similarity global score lower than 0.7 are in white, and residues with a similarity global score in the range 0.7–1.0 are color-ramped in shades of red (with dark red showing the greatest conservation)