Fig. 5.

Autocrine TL1A activates DR3. a HT29 cells were treated with or without 100 nM biotinylated diazonamide for 16 h before the conditioned cell culture media were removed for soluble TL1A analysis. A TL1A ELISA kit was used to determine the concentration of TL1A released into the media. b HT29 cells were treated as in a. Then biotinylated diazonamide was eliminated from condition medium by streptavidin agarose. The concentrated medium was applied to the HT29-DR3 cells pretreated with control IgG or TL1A neutralizing antibody. Values are presented as means ± s.d. c, d DR3 secretion is lysosome-dependent. c HT29-DR3 cells were grown in the chamber slides and treated with 100 nM taxol for the indicated time. Cells were fixed and stained with anti-TL1A and anti-LAMP2 antibodies. The slides were mounted in a medium containing DAPI to stain DNA (Scale bar: 5 μm). d HT29-DR3 cells were treated with 100 nM taxol in the presence or absence of 100 μM chloroquine for 16 h. TL1A ELISA kit was used to measure the concentrations of TL1A in the medium. *p < 0.05, ***p < 0.001