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. 2018 May 14;8:7500. doi: 10.1038/s41598-018-25775-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of LC27, LC67, and PM on Th17 and Treg differentiation in the colon of mice with TNBS-induced colitis. (A) Effects on Th17 cell differentiation. (a) Effects on Th17 cell differentiation assessed by FACS. (b) Effects on ROTγt and IL-17 expression assessed by qRT-PCR. Cells isolated from the lamina propria were stained for cell surface CD4 and intracellular IL-17 and analyzed by flow cytometry. (B) Effects on Th17 cell differentiation. (a) Effects on Th17 cell differentiation assessed by FACS. (b) Effects on Foxp3 and IL-10 expression assessed by qRT-PCR. Cells isolated from the lamina propria were stained for the cell surface CD4 and CD25 and intracellular Foxp3 and analyzed by flow cytometry. TNBS, except in the normal control group, was intrarectally administered to mice and test agents [saline, LC5, LC27, LC67, LC68 (2 × 10⁹ CFU/mouse), or sulfasalazine (SUL; 50 mg/kg)] were orally administered for 3 days. All data are shown as the mean ± SD (n = 6). All values are shown as the mean ± SD. (n = 6). ^#p < 0.05 vs. normal control group. *p < 0.05 vs. group treated with TNBS alone.