Fig. 3. NDRG3 inhibits the AKT-dependent translation of HIF-1α.

a Phospho-AKT (S473) and total AKT levels were analyzed in PC3 and DU145 cells that were transfected with siNDRG3 and incubated under hypoxia for 24 h. b The transfected PC3 or DU145 cells were pre-treated with 1 μM MK-2206 for 16 h and incubated with 15 μM MG132 for the indicated times. Western blot intensities were analyzed using ImageJ, and the relative band intensities were normalized to the corresponding β-tubulin intensities. Each symbol represents the mean ± s.d. (n = 3). c The TK-HIF1A 5′UTR-luciferase reporter and the CMV-β-galactosidase plasmids were co-transfected with siCtrl or siNDRG3 into PC3 and DU145 cells. After stabilization for 24 h, the cells were incubated with DMSO or MK-2206 under normoxia or hypoxia for 24 h. Luciferase activities (means + s.d., n = 3) were normalized to β-galactosidase activities. * denotes P < 0.05 between the indicated groups