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. 2018 May 14;50(5):61. doi: 10.1038/s12276-018-0089-y

Fig. 4. NDRG3 attenuates HIF-1-mediated HRE activation under hypoxia.

Fig. 4

a PC3 and DU145 cells were co-transfected with the HRE- or mutated HRE-luciferase plasmid, the CMV-β-galactosidase plasmid, and siCtrl or siNDRG3, and incubated under normoxia or hypoxia for 24 h. b Cells were co-transfected with the reporter, the CMV-β-galactosidase, and pcDNA or MYC-NDRG3 plasmids, and incubated under normoxia or hypoxia for 24 h. c Cells were co-transfected with the HRE reporter plasmid, the β-galactosidase plasmid, and siCtrl or siNDRG3. The cells were treated with DMSO or MK-2206 and incubated under normoxia or hypoxia for 24 h. Luciferase activities (means + s.d., n = 3) were normalized to β-galactosidase activities. * denotes P < 0.05 between the indicated groups