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. 2018 May 14;50(5):61. doi: 10.1038/s12276-018-0089-y

Fig. 5. NDRG3 represses the p300-dependent transcription of HIF-1α.

Fig. 5

a, b The MYC-NDRG3 plasmid or siNDRG3 was co-transfected with the Gal4 promoter-Luc reporter plasmid and the Gal4/DBD-HIF-1α_CAD plasmid or the Gal4/DBD-HIF-1α_CAD N803A plasmid into PC3 and DU145 cells. Cells were incubated under normoxia or hypoxia for 24 h. c The MYC-NDRG3 plasmid or siNDRG3 was co-transfected with Gal4 promoter-Luc reporter, Gal4/DBD-HIF-1α_CAD, and p300_CH1-VP16 plasmids. Cells were incubated under normoxia or hypoxia for 24 h. Luciferase activities (means + s.d., n = 3) were normalized to β-galactosidase activities and presented as relative values to the normoxic control. * denotes P < 0.05 between the indicated groups. d PC3 cells were co-transfected with GFP-HIF-1α, HA-p300, and MYC-NDRG3 (0.5 or 2 μg). Proteins in cell lysates were immunoprecipitated with an anti-HA antibody and immunoblotted using the indicated antibodies. The experiments were performed three times