Figure 1.

(A) M1 and (B) M2 mRNA expression profile of extracellular vesicle (EV)-exposed monocytes. Monocytes were purified from peripheral blood mononuclear cells of five healthy donors and were cultured unstimulated or stimulated with plasma-EVs from five other healthy donors for 0, 1, 3, and 24 h. mRNA expression was determined by RNA-Seq and analyzed longitudinally for a panel of previously described M1 and M2-associated genes. Results for EV-incubated conditions were normalized to matched, unstimulated conditions at each time point and log₂-transformed (*p < 0.05, **p < 0.01, and ***p < 0.001).