Skip to main content
. 2018 May 8;9:956. doi: 10.3389/fimmu.2018.00956

Figure 5.

Figure 5

Extracellular vesicles (EVs) in healthy controls and as predictors of mortality in intensive care unit patients. (A) Gating strategy for EVs shows detection of beads sized 100–1,000 nm on the SSC channel in the left panel, and gating of EVs in the right panel. Representative flow cytometry plots for (B) red blood cell (RBC) and (C) granulocyte markers are shown. (D) Levels of EVs were measured in 48 healthy control subjects using a panel of markers to identify EVs derived from platelets (CD41a and CD62P), RBCs (CD235a and CD108a), and endothelial cells (CD142). CD41a+ EVs were significantly more abundant than all other populations (p < 0.0001); all other significant differences are noted on the graph. (E) White blood cell (WBC)-EVs were characterized based on cell of origin (CD3, CD16, CD19, CD66b) and expression of co-stimulatory (CD28 and CD154), and adhesion molecules (CD62L, CD11b, and CD15). (F) Of 62 parameters measured at baseline in 100 critically ill subjects, the 6 associated with 28-day mortality are shown, including EVs expressing four markers (CD15, CD11b, CD62P, and CD66b) (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001).